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Title: Characterization of marine diatom-infecting virus promoters in the model diatom Phaeodactylum tricornutum
Authors: Kadono, Takashi
Miyagawa-Yamaguchi, Arisa
Kira, Nozomu
Tomaru, Yuji
Okami, Takuma
Yoshimatsu, Takamichi
Hou, Liyuan
Ohama, Takeshi
Fukunaga, Kazunari
Okauchi, Masanori
Yamaguchi, Haruo
Ohnishi, Kohei
Falciatore, Angela
Adachi, Masao
Issue Date: Dec-2015
Publisher: Nature Publishing Group
Journal Title: Scientific Reports
Volume: 5
Start Page: 18708-1
End Page: 18708-13
Abstract: Viruses are considered key players in phytoplankton population control in oceans. However, mechanisms that control viral gene expression in prominent microalgae such as diatoms remain largely unknown. In this study, potential promoter regions isolated from several marine diatom-infecting viruses (DIVs) were linked to the egfp reporter gene and transformed into the Pennales diatom Phaeodactylum tricornutum. We analysed their activity in cells grown under different conditions. Compared to diatom endogenous promoters, novel DIV promoter (ClP1) mediated a significantly higher degree of reporter transcription and translation. Stable expression levels were observed in transformants grown under both light and dark conditions, and high levels of expression were reported in cells in the stationary phase compared to the exponential phase of growth. Conserved motifs in the sequence of DIV promoters were also found. These results allow the identification of novel regulatory regions that drive DIV gene expression and further examinations of the mechanisms that control virus-mediated bloom control in diatoms. Moreover, the identified ClP1 promoter can serve as a novel tool for metabolic engineering of diatoms. This is the first report describing a promoter of DIVs that may be of use in basic and applied diatom research.
Rights: This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
Rights URI: http://creativecommons.org/licenses/by/4.0/
DOI: 10.1038/srep18708
Type: Journal Article
URI: http://hdl.handle.net/10173/1496
Appears in Collections:学術雑誌論文

Please use this identifier to cite or link to this item: http://hdl.handle.net/10173/1496

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