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Title: Effect of an Introduced Phytoene Synthase Gene Expression on Carotenoid Biosynthesis in the Marine Diatom Phaeodactylum tricornutum
Authors: Kadono, Takashi
Kira, Nozomu
Suzuki, Kengo
Iwata, Osamu
Ohama, Takeshi
Okada, Shigeru
Nishimura, Tomohiro
Akakabe, Mai
Tsuda, Masashi
Adachi, Masao
Issue Date: Aug-2015
Publisher: MDPI
Journal Title: Marine Drugs
Volume: 13
Issue: 8
Start Page: 5334
End Page: 5357
Abstract: Carotenoids exert beneficial effects on human health through their excellent antioxidant activity. To increase carotenoid productivity in the marine Pennales Phaeodactylum tricornutum, we genetically engineered the phytoene synthase gene (psy) to improve expression because RNA-sequencing analysis has suggested that the expression level of psy is lower than other enzyme-encoding genes that are involved in the carotenoid biosynthetic pathway. We isolated psy from P. tricornutum, and this gene was fused with the enhanced green fluorescent protein gene to detect psy expression. After transformation using the microparticle bombardment technique, we obtained several P. tricornutum transformants and confirmed psy expression in their plastids. We investigated the amounts of PSY mRNA and carotenoids, such as fucoxanthin and β-carotene, at different growth phases. The introduction of psy increased the fucoxanthin content of a transformants by approximately 1.45-fold relative to the levels in the wild-type diatom. However, some transformants failed to show a significant increase in the carotenoid content relative to that of the wild-type diatom. We also found that the amount of PSY mRNA at log phase might contribute to the increase in carotenoids in the transformants at stationary phase.
Rights: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
Rights URI: http://creativecommons.org/licenses/by/4.0/
DOI: 10.3390/md13085334
Type: Journal Article
URI: http://hdl.handle.net/10173/1481
Appears in Collections:学術雑誌論文

Please use this identifier to cite or link to this item: http://hdl.handle.net/10173/1481

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